>Corresponsing Author : Michel Leclerc
>Article Type : Research Article
>Volume : 1 | Issue : 1
>Received Date : 23 September, 2021
>Accepted Date : 8 October, 2021
>Published Date : 11 October, 2021
>DOI : https://doi.org/10.54289/JVVD2100102
>Citation : Leclerc M (2021) Comparisons Between 3 Invertebrates Igkappa Genes : From the Less Evolved to the Most One.Bioinformatic Data. J Virol Viral Dis 1(1). doi https://doi.org/10.54289/JVVD2100102
>Copyright : © 2021 Leclerc M. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Research Article | Open Access
Immunology of Invertebrates, Department of Biology -Biochemistry, Orléans University, France
*Corresponding author: Michel Leclerc, Immunology of Invertebrates, Department of Biology -Biochemistry, Orléans University, France
We attempt to establish, in the present paper, comparisons between 3 classes of Echinodermata (Asterids, Ophuirids, Crinoïds) out of 5 classes, at the level of genomes.
Mainly we compare the IGKappa genes of these 3 classes which belong to Invertebrates.
The Ophuirid IGKappa gene from Ophiocomina nigra seems to be the most evolved, in terms of immune functions, when compared to the two other ones.
Keywords: Invertebrates; Echinodermata; Immunoglobulins; Evolution
We recall that biosynthesis of 3 IGKappa genes have been performed [1, 2, 3].They belong to Echinodermata, first Asterids with Asterias rubens as a model of study in 2014, second Ophuirids (Ophiocomina nigra) and Crinoïds (Antedon bifida) in 2021 [2, 3].
We try now to compare them.
We performed bioinformatic assays according to the method of Marchler-Bauer [4,5] (by the use of various blasts directed against Vertebrate IG genes).
1) Antedon bifida (Crinoïds)
BlastP of the two predicted amino acids sequences.
Conserved domains on:
2) Asterias rubens: Two region features as shown below (Fig.1)
Fig.1. Predicted: Asterias rubens uncharacterized LOC117296905 (LOC117296905)
Two region features:
a. Region Ig
Length: 105 aa
Comment: U3 small nucleolar RNA-associated protein 14 (function unknown)
Length: 136 aa
3) Ophiocomina nigra:2 Immunoglobulin domains as seen below (Fig.2)
Fig.2: IGK@ protein [Homo sapiens] graphic by NCBI [Ref.]
The AAH30813.1 protein has two immunoglobulin domains:
c. Region 1
Comment: Immunoglobulin (Ig) light chain, kappa type, Variable (V) domain
Length 105 aa
1. Region 2
Comment: Immunoglobulin constant domain
Length 100 aa
As previously described, we observe something which recalls as an evolution between these3 Echinodermata (Invertebrates):
1)Antedon bifida (Crinoïds)
2)Asterias rubens (Asterids)
3)Ophiocomina nigra (Ophuirids)
The Antedon bifida IGKappa gene with its two predicted amino-acids sequence presents no Immunoglobulin site; the Asterias rubens IGKappa gene:1 Immunoglobulin Domain; the Ophiocomina nigra IGKappa gene: 2 Immunoglobulin Domains at the level of constant part and the variable part of Immunoglobulins (entiere homology with human IGK) 
At our knowledge it seems highly important to give that data in terms of emergence of Immunoglobulins in Invertebrates when compared to Vertebrates.